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Cloning and Expression
Enzymes
ClaI
Supplier Images
ClaI
ClaI from New England Biolabs
Be the first to write a review!
Citations:
(31)
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Supplier Page from
New England Biolabs for
ClaI
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
ClaI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0197L
Concentration
5 u/µL
Quantity
5000 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
AT/CGAT
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (31)
(1)
Cytoglobin regulates NO-dependent cilia motility and organ laterality during development
Nat Commun
December 14, 2023
Elizabeth R. Rochon, Jianmin Xue, Manush Sayd Mohammed, Caroline Smith, Anders Hay-Schmidt, Anthony W. DeMartino, Adam Clark, Qinzi Xu, Cecilia W. Lo, Michael Tsang,
et al.
the forward primer and an Xho1 site to the reverse primer. pCS2+ plasmid was digested with
ClaI
(
NEB
,
R0197s
) and Xho1 (
NEB
,
R0146s
) and ligated with the cygb2
PCR product
. nos2b mRNA overexpression plasmid was
(2)
Biomolecular condensates in fungi are tuned to function at specific temperatures
bioRxiv
November 30, 2023
Benjamin M. Stormo, Grace A. McLaughlin, Logan K. Frederick, Ameya P. Jalihal, Sierra J Cole, Ian Seim, Fred S. Dietrich, Amy S. Gladfelter
verified by
Sanger sequencing
(Genewiz, South Plainfield, NJ, USA). Plasmids were linearized using
NcoI
(
R0197
, R3193,
New England BioLabs
, Waltham, MA, USA) at 37°C for 1 hr. Linearization was confirmed by running
(3)
Doxycycline-inducible Expression of Proteins at Near-endogenous Levels in Mammalian Cells Using the Sleeping Beauty Transposon System
BIO-PROTOCOL
October 20, 2023
Karolina Zak, Costin N. Antonescu
reagents Restriction enzymes, e.g.,
NcoI
and
ClaI
(
New England Biolabs
, catalog numbers:
R0193L
and
R0197L
, respectively)Competent DH5α
E. coli
(
Thermo Fisher
, catalog number:
18265017
)D-
MEM
/F-12 (1×), liquid
(4)
Increased prime edit rates in KCNQ2 and SCN1A via single nicking all-in-one plasmids
BMC Biology
July 13, 2023
N. Dirkx, Wout J. Weuring, E. De Vriendt, N. Smal, J. van de Vondervoort, Ruben van ’t Slot, M. Koetsier, N. Zonnekein, Tim De Pooter, S. Weckhuysen,
et al.
annealed following previous described protocol. Digestion of the backbone was performed by
ClaI
(
NEB
#
R0197L
) according to manufacturer’s instructions.
Ligation
of the annealed oligos in the linear backbone was
(5)
Coxsackievirus B3 infects and disrupts human induced-pluripotent stem cell derived brain-like endothelial cells
Frontiers in Cellular and Infection Microbiology
April 17, 2023
Julia Mamana, Gabrielle M. Humber, Eric R. Espinal, Soojung Seo, Nadine Vollmuth, Jon Sin, Brandon J. Kim
These constructs were then linearized by digesting with
ClaI
restriction enzyme (
New England BioLabs
;
R0197S
) and subsequently used for
in vitro transcription
using the mMESSAGE mMACHINE kit (
Thermo Fisher
;
AM1344
).
(6)
Widespread allele-specific topological domains in the human genome are not confined to imprinted gene clusters
Genome Biology
March 3, 2023
Stephen Richer, Yuan Tian, Stefan Schoenfelder, Laurence Hurst, Adele Murrell, Giuseppina Pisignano
Hi-C marking and Hi-C
ligation
efficiency was verified by
PCR
digest
assay
using
MBOI
and
CLAI
(
NEB
,
R0197S
) enzymes. Biotin is then removed from the ends of un-ligated fragments using the exonuclease properties
(7)
Production of human translation-competent lysates using dual centrifugation
RNA Biology
December 31, 2022
Lukas-Adrian Gurzeler, Jana Ziegelmüller, Oliver Mühlemann, Evangelos D. Karousis
for pCRII-hRLuc-200bp-A80, pCRII-p2luc-UAA-A80 and pCRII_SC-LD_3xFLAG-HBB, and
ClaI
;
NEB
, Cat. No.
R0197S
for pCRII_SC-LD_SMG6-3xFLAG_A80) in 1x
CutSmart buffer
(
NEB
, Cat. No.
B7204S
). The digest was monitored
(8)
Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneity
Cell reports
December 6, 2022
Kaserman JE, Werder RB, Wang F, Matte T, Higgins MI, Dodge M, Lindstrom-Vautrin J, Bawa P, Hinds A, Bullitt E,
et al
.
"
Trypsin-EDTA (0.25%), phenol red
",
ThermoFisher Scientific
,
25200114
ClaI
restriction enzyme,
New England BioLabs
,
R0197
N-2 Supplement,
ThermoFisher Scientific
,
17502048
"
B27
Supplement, minus vitamin A",
ThermoFisher Scientific
,
12587010
Ascorbic
(9)
Generation of a bovine cell line for gene engineering using an HIV-1-based lentiviral vector
Scientific Reports
October 18, 2022
Nanami Morizako, Erika P. Butlertanaka, Yuri L. Tanaka, Honoka Shibata, Tamaki Okabayashi, Hirohisa Mekata, Akatsuki Saito
V86M or H87Q were mixed with the psPAX2-IN/HiBiT plasmid, which was predigested with
ClaI
(
NEB
, Cat#
R0197L
) and
EcoRV-HF
(
NEB
, Cat#
R3195L
), and then ligated with the In-Fusion HD Cloning Kit. After the mini
(10)
Light chain 2 is a Tctex-type related axonemal dynein light chain that regulates directional ciliary motility in Trypanosoma brucei
PLOS Pathogens
September 26, 2022
Subash Godar, James Oristian, Valerie Hinsch, Katherine Wentworth, Ethan Lopez, Parastoo Amlashi, Gerald Enverso, Samantha Markley, Joshua Daniel Alper
from Meredith Morris, Clemson University, SC) from which we excised the Pex13.2 fragment with
ClaI
(
R0197S
,
New England Biolabs
, MA) and
EcoRI
restriction enzymes (
R3101S
,
New England Biolabs
, MA). We used the
(11)
Regulation of Nodal signaling propagation by receptor interactions and positive feedback
eLife
September 23, 2022
Hannes Preiß, Anna C Kögler, David Mörsdorf, Daniel ÄŒapek, Gary H Soh, Katherine W Rogers, Hernán Morales-Navarrete, MarÃa Almuedo-Castillo, Patrick Müller
recombinant protein",
Alt-R S.p. Cas9 Nuclease V3
,
IDT
,
1081058
, "Peptide, recombinant protein",
ClaI
-HF,
NEB
,
R0197
, "Peptide, recombinant protein",
EcoRI-HF
,
NEB
,
R3101
, "Peptide, recombinant protein",Exonuclease I (ExoI),
NEB
,
M0568
, "Peptide,
(12)
LRH ‐1/ NR5A2 interacts with the glucocorticoid receptor to regulate glucocorticoid resistance
EMBO reports
September 5, 2022
Svenja Michalek, Thomas Goj, Anna Pia Plazzo, Blerim Marovca, Beat Bornhauser, Thomas Brunner
P4864
Puromycin.2HCl
,
Enzo Life Sciences
,Cat#
BML‐GR312
Restriction enzyme:
ClaI
,
New England BioLabs
,Cat#
R0197
Restriction enzyme:
EcoRI
,
New England BioLabs
,Cat#
R3101
Restriction enzyme:
XbaI
,
New England BioLabs
,Cat#
(13)
Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model
Cell reports
May 31, 2022
Cheng Y, Medina A, Yao Z, Basu M, Natekar JP, Lang J, Sanchez E, Nkembo MB, Xu C, Qian X,
et al
.
Immobilon Western chemiluminescent HRP substrate
,
Millipore
,Cat#
WBKLS0500
Sp6 RNA polymerase
,
NEB
,Cat#
M0207S
SacI-HF
,
NEB
,Cat#
R3156S
ClaI
,
NEB
,Cat#
R0197S
AatII
,
NEB
,Cat#
R0117S
BsHII,
NEB
,Cat#
R0199S
Human brain microvascular endothelial cell lysate,
ScienCell
,Cat#
1006
Sodium
(14)
Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication
Molecular cell
May 5, 2022
Hsu JC, Laurent-Rolle M, Pawlak JB, Xia H, Kunte A, Hee JS, Lim J, Harris LD, Wood JM, Evans GB,
et al
.
,
Zymo Research
,Cat#
D4201
MEGAscript T7 Transcription Kit
,
Thermo Fisher Scientific
,Cat#
AMB13345
ClaI
,
New England Biolabs
,Cat#
R0197S
EcoRI-HF
,
New England Biolabs
,Cat#
R3101S
Bovine Serum Albumin (BSA),American Bioanalytical,Cat#AB01088
TRIzol Reagent
(15)
A Zika virus mutation enhances transmission potential and confers escape from protective dengue virus immunity
Cell Reports
April 12, 2022
Jose Angel Regla-Nava et al.
(
New England BioLabs
),Cat. #
R0182S
NaeI
,(
New England BioLabs
),Cat. #
R0190S
ClaI
,(
New England BioLabs
),Cat. #
R0197S
mMESSAGE mMACHINE™ T7 transcription kit
,(
Thermo Fisher Scientific
),Cat. #
AM1344
Complement-inactivated
(16)
Structure-guided changes at the V2 apex of HIV-1 clade C trimer enhance elicitation of autologous neutralizing and broad V1V2-scaffold antibodies
Cell Reports
March 1, 2022
Anusmita Sahoo et al.
T4 Polynucleotide Kinase
,
NEB
,Cat#
M0201S
DpnI
,
NEB
,Cat#
R0176S
NheI-HF
,
NEB
,Cat#
R3131S
ClaI
,
NEB
,Cat#
R0197S
Galanthus nivalis
lectin-agarose,
Vector Laboratories
,Cat#
AL-1243-5
Expifectamine™ 293 transfection kit
(17)
Selection-free endogenous tagging of cell lines by bicistronic co-expression of the surface antigen NGFR
MethodsX
January 1, 2022
Seibert M, Kurrle N, Stolp V, Nürnberger H, Tzschentke S, Börner L, Wempe F, Serve H, Schnütgen F.
vectors:•Restriction enzyme (
BsmBI-v2
) (
New England Biolabs
, #
R0739L
)•Restriction enzyme (
ClaI
) (
New England Biolabs
, #
R0197S
)•Restriction enzyme (
XmaI
) (
New England Biolabs
, #
R0180S
)•
Antarctic Phosphatase
(
New England Biolabs
,
(18)
Production of single- and multiple-gene-modified mice via maternal SpCas9-based gene editing
STAR Protocols
June 18, 2021
Takayuki Sakurai, Takayuki Shindo
(
Integrated DNA Technologies
),Cat#
1081058
Calcium chloride dihydrate (CaCl2・2H2O),Merck,Cat# C7902 Cla I,
New England Biolabs
,Cat#
R0197
D(+)-Glucose
,
Merck
,Cat#
G7021
-100G DPBS,
Thermo Fisher
,Cat#
14190144
EDTA
・2Na,FUJIFILM Wako,Cat# 343-01861 Gelatin,Merck,Cat#
(19)
A Rab escort protein regulates the MAPK pathway that controls filamentous growth in yeast
Scientific Reports
December 17, 2020
Sheida Jamalzadeh, Atindra N. Pujari, Paul J. Cullen
PCR product
and pGAD-C1 vector were digested with
ClaI
(5′-ATCGAT-3′,
New England BioLabs
Inc., MA, CA#
R0197S
) and
SalI
(5′-GTCGAC-3′,
New England Biolabs
Inc., MA, CA#
R3138S
) restriction enzymes. Digested insert
(20)
MeCP2 links heterochromatin condensates and neurodevelopmental disease
Nature
October 1, 2020
Li CH, Coffey EL, Dall'Agnese A, Hannett NM, Tang X, Henninger JE, Platt JM, Oksuz O, Zamudio AV, Afeyan LK,
et al
.
T1030S
). Methylation of templates was verified by methyl-specific restriction digestion using
ClaI
(
NEB
R0197S
). Poly-
nucleosome
purification
Poly-
nucleosome
arrays were purified from
mESCs
using a modified protocol
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ClaI from New England Biolabs
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